推薦培養(yǎng)基:(FM, Cat. No. 2301)
儲(chǔ)存：液氮
運(yùn)輸：干冰
用途：科研
The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some
distinguishing features, for examle, they have long branching processes and gap junctions. Their principle
function is production of type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar
septa. Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury.
The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury
[1]. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For
examle, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the
development of hypoxia-induced pulmonary hypertension [2].
HPF from ScienCell Research Laboratories are isolated from human lung tissue. HPF are cryopreserved at primary
culture and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HPF are characterized by
immunofluorescent method with antibody to fibronectin. HPF are negative for HIV-1, HBV, HCV, mycoplasma,
bacteria, yeast and fungi. HPF are guaranteed to further expand for 15 population doublings at the conditions
provided by ScienCell Research Laboratories.
Reference
[1] Kuwano K, Hagimoto N, Hara N. (2001) Molecular mechanisms of pulmonary fibrosis and current treatment.
Curr Mol Med 1(5):551-73.
[2] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2004) Selective expansion of fibroblast subpopulations from
pulmonary artery adventitia in response to hypoxia. Am J Physiol Lung Cell Mol Physiol 282(5):L976-86
